phosphorylated nf-κb p65 (nf-κb p-p65) antibody  (Cell Signaling Technology Inc)

Journal: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology

Article Title: SIRT5 regulates granulosa cell proliferation and apoptosis in polycystic ovarian syndrome via desuccinylation of GLI1.

doi: 10.1080/09513590.2025.2515516

Figure Lengend Snippet: Figure 3. Silencing of SIRT5 activates the sonic hedgehog (SHH) signaling path way. Immunoblotting was performed to detect protein levels of the (A) β-catenin in the wnt/β-catenin pathway, (B) SHH, SMO, GLI1 in the SHH pathway, and (C) NF-κB p65, p- NF-κB p65, IkBα, p-IkBα in the NF-κB pathway.

Article Snippet: Primary antibodies were shown below: anti-CPT1A (66039-1- Ig, 1:10000, Proteintech), anti-KAT2A (66575-1-Ig, 1:5000, Proteintech), anti-KAT3B (20695-1-AP, 1:1000, Proteintech), anti-SIRT5 (67257-1-Ig, 1:5000, Proteintech), anti-SIRT7 (12994-1-AP, 1:3000, Proteintech), anti-bcl-2 (60178-1-Ig, 1:2000, Proteintech, Wuhan, China), anti-bax (60267-1-Ig, 1:5000, Proteintech), anti-cleaved caspase-3 (25128-1-AP, 1:1000, Proteintech), anti-β-catenin (66379-1-Ig, 1:5000, Proteintech), anti-SHH (20697-1-AP, 1:2000, Proteintech), anti-SMO (ab236465, 1:1000, Abcam, Cambridge, UK), anti-GLI1 (GLI1, 1:5000, Proteintech), anti-NF-κB p65 (66535-1-Ig, 1:3000, Proteintech), anti-phosphorylated (p)-NF-κB p65 (82335-1-RR, 1:5000, Proteintech), anti-IkBα (66418-1-Ig, 1:10000, Proteintech), and anti-p-IkBα (82349-1-RR, 1:10000, Proteintech), anti-succinyllysine (suc; PTM-401, 1:1000, PTMBIO, Hangzhou, China), and anti-GAPDH (60004-1-Ig, 1:50000, Proteintech).

Techniques: Western Blot

Journal: World Journal of Clinical Oncology

Article Title: Natural compound rosmarinic acid displays anti-tumor activity in colorectal cancer cells by suppressing nuclear factor-kappa B signaling

doi: 10.5306/wjco.v16.i5.105341

Figure Lengend Snippet: Rosmarinic acid inhibits nuclear factor-kappa B signaling in colorectal cancer cells. A: Molecular modeling of rosmarinic acid (RA)/inhibitory kappa B kinase beta complex. 3D presentation of the molecular docking complex pose (I), 2D presentation (II), and 3D presentation (III) of the interactions between RA and the key residues of inhibitory kappa B kinase beta; B and C: HT29 (B) and SW480 (C) cells transfected with p-nuclear factor-kappa B-Luc along with Renilla luciferase were incubated with indicated RA overnight, followed by luciferase assay; D: HT29 and SW480 cells were incubated with increasing concentrations of RA for 24 hours, followed by Western blot analysis using antibodies against p-p65 and nuclear factor-kappa B P65. GAPDH was used as a loading control; E: Starved HT29 cells were treated with indicated RA for 12 hours, and then cells were incubated with 200 ng/mL lipopolysaccharide for 30 minutes, followed by Western blot analysis using antibodies against p-p65 and GAPDH; F and G: HT29 cells were incubated with increasing concentrations of RA for 24 hours, followed by quantitative real-time polymerase chain reaction to evaluate the mRNA levels of cyclin D1 (F) and MYC (G). GAPDH was used as an internal control. n = 3. Data are shown as the mean ± SD. a P < 0.001 vs group ‘0’, b P < 0.0001 vs group ‘0’. RA: Rosmarinic acid; NF-κB: Nuclear factor-kappa B; LPS: Lipopolysaccharide.

Article Snippet: Anti-phosphorylated nuclear factor-kappa B (NF-κB) P65 (p-p65, Ser536) antibody, anti-NF-κB P65 antibody, anti-Bcl-2 antibody, anti-caspase-3 antibody, and anti-phosphorylated protein kinase B (p-AKT, Ser473) antibody were purchased from Cell Signaling Technology, Danvers, MA, United States.

Techniques: Transfection, Luciferase, Incubation, Western Blot, Control, Real-time Polymerase Chain Reaction